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91.
Spector AA Deo N Grosh K Ratnanather JT Raphael RM 《The Journal of membrane biology》2006,209(2-3):135-152
The outer hair cell (OHC) is an extremely specialized cell and its proper functioning is essential for normal mammalian hearing.
This article reviews recent developments in theoretical modeling that have increased our knowledge of the operation of this
fascinating cell. The earliest models aimed at capturing experimental observations on voltage-induced cellular length changes
and capacitance were based on isotropic elasticity and a two-state Boltzmann function. Recent advances in modeling based on
the thermodynamics of orthotropic electroelastic materials better capture the cell’s voltage-dependent stiffness, capacitance,
interaction with its environment and ability to generate force at high frequencies. While complete models are crucial, simpler
continuum models can be derived that retain fidelity over small changes in transmembrane voltage and strains occurring in
vivo. By its function in the cochlea, the OHC behaves like a piezoelectric-like actuator, and the main cellular features can
be described by piezoelectric models. However, a finer characterization of the cell’s composite wall requires understanding
the local mechanical and electrical fields. One of the key questions is the relative contribution of the in-plane and bending
modes of electromechanical strains and forces (moments). The latter mode is associated with the flexoelectric effect in curved
membranes. New data, including a novel experiment with tethers pulled from the cell membrane, can help in estimating the role
of different modes of electromechanical coupling. Despite considerable progress, many problems still confound modelers. Thus,
this article will conclude with a discussion of unanswered questions and highlight directions for future research. 相似文献
92.
W Richard Mukabana Khadija Kannady G Michael Kiama Jasper N Ijumba Evan M Mathenge Ibrahim Kiche Gamba Nkwengulila Leonard Mboera Deo Mtasiwa Yoichi Yamagata Ingeborg van Schayk Bart GJ Knols Steven W Lindsay Marcia Caldas de Castro Hassan Mshinda Marcel Tanner Ulrike Fillinger Gerry F Killeen 《Malaria journal》2006,5(1):1-14
Background
Malaria imposes significant costs on households and the poor are disproportionately affected. However, cost data are often from quantitative surveys with a fixed recall period. They do not capture costs that unfold slowly over time, or seasonal variations. Few studies investigate the different pathways through which malaria contributes towards poverty. In this paper, a framework indicating the complex links between malaria, poverty and vulnerability at the household level is developed and applied using data from rural Kenya.Methods
Cross-sectional surveys in a wet and dry season provide data on treatment-seeking, cost-burdens and coping strategies (n = 294 and n = 285 households respectively). 15 case study households purposively selected from the survey and followed for one year provide in-depth qualitative information on the links between malaria, vulnerability and poverty.Results
Mean direct cost burdens were 7.1% and 5.9% of total household expenditure in the wet and dry seasons respectively. Case study data revealed no clear relationship between cost burdens and vulnerability status at the end of the year. Most important was household vulnerability status at the outset. Households reporting major malaria episodes and other shocks prior to the study descended further into poverty over the year. Wealthier households were better able to cope.Conclusion
The impacts of malaria on household economic status unfold slowly over time. Coping strategies adopted can have negative implications, influencing household ability to withstand malaria and other contingencies in future. To protect the poor and vulnerable, malaria control policies need to be integrated into development and poverty reduction programmes. 相似文献93.
Barlow MA Deo S Johnson S Caffrey JL 《Experimental biology and medicine (Maywood, N.J.)》2006,231(4):387-395
This study examined the hypothesis that vagotonic and sympatholytic effects of cardiac enkephalins are independently mediated by different receptors. A dose-response was constructed by administering the delta-receptor opioid methionine-enkephalin-arginine-phenylalanine (MEAP) by microdialysis into the interstitium of the canine sinoatrial node during vagal and sympathetic stimulation. The right cardiac sympathetic nerves were stimulated as they exited the stellate ganglion at frequencies selected to increase heart rate approximately 35 bpm. The right cervical vagus was stimulated at frequencies selected to produce a two-step decline in heart rate of 25 and 50 bpm. A six-step dose-response was constructed by recording heart rates during nerve stimulation as the dose of MEAP was increased between 0.05 pmol/min and 1.5 nmol/min. Vagal transmission improved during MEAP at 0.5 pmol/min. However, sympathetically mediated tachycardia was unaltered with any dose of MEAP. In Study 2, a similar dose-response was constructed with the kappa-opioid receptor agonist trans(+/-)-3-4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl]benzeneacetamide-HCl (U-50488H) to illustrate an independent sympatholytic effect and to verify its kappa-receptor character. U-50488H gradually suppressed the sympathetic tachycardia, with a significant effect obtained only at the highest dose (1.5 nmol/min). U-50488H had no effect on vagally mediated bradycardia. Surprisingly, the sympatholytic effect was not reversed by withdrawing U-50488H or by the subsequent addition of the kappa-antagonist 17,17'-(dichloropropylmethyl)-6,6',7,7'-6,6'-imino-7,7'-binorphinan-3,4',14,14'-tetroldi-hydrochloride (norBNI). Study 3 was conducted to determine whether the sympatholytic effect of U-50488H could be prevented by norBNI. NorBNI blocked the sympatholytic effect of the U50488H for 90 mins. When norBNI was discontinued afterward and U-50488H was continued alone, a sympatholytic effect emerged within 30 mins. Collectively these observations support the hypothesis that the vagotonic influence of MEAP is not dependent on a sympatholytic influence. Furthermore, the sympatholytic effect is mediated independently by kappa-receptors. The sympatholytic effect of sustained kappa-receptor stimulation appears to evolve gradually into a functional state not easily reversed. 相似文献
94.
95.
We have limited understanding of how tropical canopy foliage varies along environmental gradients, and how this may in turn affect forest processes and functions. Here, we analyse the relationships between canopy leaf area index (LAI) and above ground herbaceous biomass (AGBH) along environmental gradients in a moist forest and miombo woodland in Tanzania. We recorded canopy structure and herbaceous biomass in 100 permanent vegetation plots (20 m × 40 m), stratified by elevation. We quantified tree species richness, evenness, Shannon diversity and predominant height as measures of structural variability, and disturbance (tree stumps), soil nutrients and elevation as indicators of environmental variability. Moist forest and miombo woodland differed substantially with respect to nearly all variables tested. Both structural and environmental variables were found to affect LAI and AGBH, the latter being additionally dependent on LAI in moist forest but not in miombo, where other factors are limiting. Combining structural and environmental predictors yielded the most powerful models. In moist forest, they explained 76% and 25% of deviance in LAI and AGBH, respectively. In miombo woodland, they explained 82% and 45% of deviance in LAI and AGBH. In moist forest, LAI increased non-linearly with predominant height and linearly with tree richness, and decreased with soil nitrogen except under high disturbance. Miombo woodland LAI increased linearly with stem density, soil phosphorous and nitrogen, and decreased linearly with tree species evenness. AGBH in moist forest decreased with LAI at lower elevations whilst increasing slightly at higher elevations. AGBH in miombo woodland increased linearly with soil nitrogen and soil pH. Overall, moist forest plots had denser canopies and lower AGBH compared with miombo plots. Further field studies are encouraged, to disentangle the direct influence of LAI on AGBH from complex interrelationships between stand structure, environmental gradients and disturbance in African forests and woodlands. 相似文献
96.
Deo R. Singh Fozia Ahmed Christopher King Nisha Gupta Matt Salotto Elena B. Pasquale Kalina Hristova 《The Journal of biological chemistry》2015,290(45):27271-27279
The EphA2 receptor tyrosine kinase promotes cell migration and cancer malignancy through a ligand- and kinase-independent distinctive mechanism that has been linked to high Ser-897 phosphorylation and low tyrosine phosphorylation. Here, we demonstrate that EphA2 forms dimers in the plasma membrane of HEK293T cells in the absence of ephrin ligand binding, suggesting that the current seeding mechanism model of EphA2 activation is incomplete. We also characterize a dimerization-deficient EphA2 mutant that shows enhanced ability to promote cell migration, concomitant with increased Ser-897 phosphorylation and decreased tyrosine phosphorylation compared with EphA2 wild type. Our data reveal a correlation between unliganded dimerization and tumorigenic signaling and suggest that EphA2 pro-tumorigenic activity is mediated by the EphA2 monomer. Thus, a therapeutic strategy that aims at the stabilization of EphA2 dimers may be beneficial for the treatment of cancers linked to EphA2 overexpression. 相似文献
97.
This article reports a new application of material and energy accounting techniques to characterize and quantify the relationships between material input (and the related energy flows and emissions) and the services provided (i.e., material input per service unit [MIPS]) at the neighborhood level. The case study focuses on China's small residential district (SRD). It is concluded that linking a service (in this case, residential function) enabled by a given product (neighborhood development) to the amount of materials, energy, and emissions used or produced in creating that product offers a potential way to reduce the environmental impact of that service through more efficient use of materials, enlarged service scales, and improved buying decisions. 相似文献
98.
Zhao-Jun Liu Pirouz Daftarian Letícia Kovalski Bo Wang Runxia Tian Diego M. Castilla Emre Dikici Victor L. Perez Sapna Deo Sylvia Daunert Omaida C. Velazquez 《PloS one》2016,11(4)
Stem cell therapy has emerged as a promising approach for treatment of a number of diseases, including delayed and non-healing wounds. However, targeted systemic delivery of therapeutic cells to the dysfunctional tissues remains one formidable challenge. Herein, we present a targeted nanocarrier-mediated cell delivery method by coating the surface of the cell to be delivered with dendrimer nanocarriers modified with adhesion molecules. Infused nanocarrier-coated cells reach to destination via recognition and association with the counterpart adhesion molecules highly or selectively expressed on the activated endothelium in diseased tissues. Once anchored on the activated endothelium, nanocarriers-coated transporting cells undergo transendothelial migration, extravasation and homing to the targeted tissues to execute their therapeutic role. We now demonstrate feasibility, efficacy and safety of our targeted nanocarrier for delivery of bone marrow cells (BMC) to cutaneous wound tissues and grafted corneas and its advantages over conventional BMC transplantation in mouse models for wound healing and neovascularization. This versatile platform is suited for targeted systemic delivery of virtually any type of therapeutic cell. 相似文献
99.
Samson Aman Samson Kiswaga John Richard Mbwambo Deo Shirima Ahmed S. Mndolwa Urs Schaffner Ren Eschen 《Ecology and evolution》2020,10(11):5034-5044
There is insufficient information regarding the factors affecting the environmental impacts of alien species. In particular, little is known about whether there is any relationship between the invasiveness (establishment and spread) of an introduced species and its per capita impact. We experimentally assessed the relationship between the extent of spread of up to 29 alien plant species and their impact on recruitment of native tree species in Amani Botanical Garden, Tanzania. We also studied the effects of allelochemicals of selected alien on native plant species to assess potential mechanisms of impact. We found no relationship between the extent of spread of an alien tree species and their impact on seed germination, seedling survival, and seedling communities of native trees in their understory, and no indication that allelochemicals consistently explain their effects on recruitment of the studied species. These results suggest that extent of spread cannot be used as a proxy for impact. Hence, managers should continue assessing both the spread and the impact of alien species when prioritizing alien species for management. 相似文献
100.
Mj-AMP2, a knottin-type antimicrobial peptide, in vitro inhibits the growth of several plant pathogenic fungi including Magnaporthe oryzae. We demonstrate that transgenic rice (Oryza sativa L.) plants expressing the Mj-AMP2 gene show enhanced resistance to M. grisea, the causal agent of the rice blast disease. Mj-AMP2 was efficiently expressed and the level of Mj-AMP2 ranged from 0.32% to 0.38% of the total protein in the transgenic rice plants. In vitro inhibitory activity assays with the crude protein extract from transgenic rice indicated that the Mj-AMP2 protein produced was biologically active. Constitutive expression of Mj-AMP2 in transgenic rice reduces the growth of M. grisea by 63% with respect to untransformed control plant, and no effect on plant phenotype was observed. Transgene expression of Mj-AMP2 gene was not accompanied by an induction of pathogenesis-related (PR) gene expression indicating that the transgene product itself is directly active against the pathogen. The results presented in this study suggest that the Mj-AMP2 gene could be a useful candidate for protection of rice plants against the rice blast fungus M. grisea. 相似文献